Kan'in Tokkyuu: Michi Shio

Judging by the plot of this anime, there is a long-distance train in Japan, where one of the employees, perhaps not quite of the usual orientation, collects girls among the passengers and conducts various non-standard actions with them in a special carriage designed for such purposes. His assistant also works on the train, who may also be prone to non-standard relationships, with whom he conducts experiments from time to time.